Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Immunological disruption of antiangiogenic signals by recruited allospecific T cells leads to corneal allograft rejection.

doi: 10.4049/jimmunol.1103216

Figure Lengend Snippet: FIGURE 1. Corneal graft survival curves of BALB/c mice correlated with the endostatin kinetics of the grafts. (A) Corneal graft survival curves (n = 12). All syngeneic corneal grafts survived (BALB/c [H-2d] to BALB/c [H-2d]), whereas 75% of allografts (C57BL/6 [H-2b] to BALB/c [H-2d]) were rejected between POD20 and POD60. (B) Kinetics of endostatin production. After corneal transplantation, upregulation of endostatin production occurred in both allogeneic and syngeneic transplants. Although syngeneic transplants retained high endostatin levels, endostatin production in allogeneic transplants started to decline after POD10 and was significantly decreased by POD20. *p , 0.05. (C) Kinetics of VEGF production. VEGF expression increased in both groups after transplantation, began decreasing after POD3, and increased again after POD10. Allografts retained higher VEGF production compared with syngeneic grafts (p , 0.05). (D) DiI staining identified a significant number of blood vessels in allogeneic grafts, whereas no vessels were detected in syngeneic grafts at POD40. Original magnification 35.

Article Snippet: Recombinant mouse endostatin (R&D Systems) was used as the standard protein.

Techniques: Transplantation Assay, Expressing, Staining

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Immunological disruption of antiangiogenic signals by recruited allospecific T cells leads to corneal allograft rejection.

doi: 10.4049/jimmunol.1103216

Figure Lengend Snippet: FIGURE 2. Immunohistochemical analysis of endostatin expression and T cell infiltration. Upper panels, Endostatin staining showed that endostatin production in syngeneic grafts re- mained elevated, and no infiltrating T cells were detected. Lower panels, In allogeneic grafts, endostatin production peaked at POD10; con- currently, T cell recruitment into the allograft started around POD10 and increased thereafter, with concomitant graft rejection. Infiltrating T cells in the allografts surrounded the endo- statin-expressing cells. As the number of T cells increased, the number of endostatin-producing cells decreased. The tissue orientation shows the epithelium at the top and the endothelium at the bottom. Images are representative of three mice/group. Scale bar, 50 mm.

Article Snippet: Recombinant mouse endostatin (R&D Systems) was used as the standard protein.

Techniques: Immunohistochemical staining, Expressing, Staining

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Immunological disruption of antiangiogenic signals by recruited allospecific T cells leads to corneal allograft rejection.

doi: 10.4049/jimmunol.1103216

Figure Lengend Snippet: FIGURE 5. Endostatin suppressed the neovascularization of the corneal allografts. (A) Real-time PCR showed that there was more relative mRNA expression of VEGFR2 in the allografts treated with PBS in comparison with allografts treated with endostatin. (B) Immunohistochemical staining showed that fewer CD31+ cells concentrated in the corneal stroma in the endostatin- treated group compared with the PBS-treated group (n = 3 mice/group). (C) DiI staining of blood vessels demonstrates that PBS-treated allografts had significant staining of vessels in the host and graft (left panel), whereas fewer vessels originating from the limbus were seen in the endostatin-treated group, in which the vessels extended to the graft margin but did not extend beyond it into the grafts (right panel). Original magnification 35.

Article Snippet: Recombinant mouse endostatin (R&D Systems) was used as the standard protein.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Comparison, Immunohistochemical staining, Staining

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Immunological disruption of antiangiogenic signals by recruited allospecific T cells leads to corneal allograft rejection.

doi: 10.4049/jimmunol.1103216

Figure Lengend Snippet: FIGURE 6. T cell infiltration decreased in endostatin-treated corneal allografts at POD40. (A and B) Real-time PCR analysis showed increased CD4 and CD8 expression in the PBS-treated group compared with the endostatin-treated group. (C and D) Immunostaining of T cells showed that there were more CD4+ and CD8+ T cells infiltrated into the corneal stroma of the PBS-treated group compared with the endostatin-treated group (n = 3 mice/group). (E and F) DAPI nuclear staining of corneal grafts. The stroma of endostatin-treated allografts maintained its structural integrity, whereas PBS-treated allografts had thicker and structurally disorganized stroma. Original magnification 320.

Article Snippet: Recombinant mouse endostatin (R&D Systems) was used as the standard protein.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Immunostaining, Staining